Composite

Part:BBa_K734003:Design

Designed by: Razan Alnahhas   Group: iGEM12_Austin_Texas   (2012-09-25)

CRE regulated by LasR quorum sensing pathway


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3566
    Illegal XbaI site found at 6100
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3566
    Illegal NheI site found at 2412
    Illegal NheI site found at 5258
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3566
    Illegal BamHI site found at 2878
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3566
    Illegal XbaI site found at 6100
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3566
    Illegal XbaI site found at 6100
    Illegal NgoMIV site found at 1898
    Illegal AgeI site found at 1701
    Illegal AgeI site found at 2564
    Illegal AgeI site found at 4076
    Illegal AgeI site found at 5344
    Illegal AgeI site found at 5668
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequences design brought together 6 parts from different plasmids and a plasmid backbone, so piecing them together in the appropriate order took time and needed high concentrations of each part.


Source

Parts were obtained from iGEM Registry, and pACYC, PQL269, and pET21 plasmids.

References